Two antipeptide antibodies were generated in chickens against sequences shared between the mouse (P24529) and human (P07101) gene products. Antibodies were affinity-purified and then mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.

Volume: 1000 µL

Concentration: 200 µg/mL

Clonality: Polyclonal

Host Species: Chicken

Species Reactivity:Human, Mouse, Rat

Applications: ICC, IHC, WB

Protein Name / Synonyms: Tyrosine 3-monooxygenase (EC 1.14.16.2) (Tyrosine 3-hydroxylase) (TH)

Target Description: Human TYH (EC 1.14.16.2) is a 58,523 dalton protein (528 amino acids) responsible for the enzymatic conversion of L-tyrosine to L-DOPA (dihydroxyphenylalanine). This enzyme is expressed in all catecholaminergic neurons of the CNS and PNS. In the CNS, TYH-positive neurons can be found within the substantia nigra, ventral tegmental area, locus ceruleus, and hypothalamus. In the PNS, TYH-positive neurons can be found within the sympathetic chain, pre-vertebral ganglia and the adrenal medulla.

Gene ID: TH TYH

Antibody Registry ID (RRID): AB_10013440

Physical State: Liquid

Production Notes: Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Tyrosine Hydroxylase gene product, but were shared between the human (P07101, NCBI) and mouse (P24529, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of both affinity-purified anti-peptide antibody preparations were mixed, and the preparation was filter-sterilized.

Validation and Application Notes

Molecular Weight: 60 kDa

Western Blot Dilution Range: 1:1000-1:2000

IHC Dilution Range: 1:1000-1:2000

Quality Control

Both of the antibodies were analyzed by immunohistochemistry (at a concentration of 3 µg/mL) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.

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