Details

Chickenswereimmunizedwithasyntheticpeptide/keyholelimpethemocyanin(KLH)conjugate.ThepeptidecorrespondedtoaregionoftheCNPasegeneproductsharedbetweenthehuman(NP_149124,NCBI)andmouse(P16330,NCBI)sequences.Afterrepeatedinjections,immuneeggswerecollected,theIgYfractionswerepurifiedfromtheyolks,andthenaffinity-purifiedusingapeptidecolumn.Theconcentrationsoftheeluateswerethenadjustedto100µg/mL,andthepreparationwasfilter-sterilized.

Volume:1000µL

Concentration:100µg/mL

Clonality:Polyclonal

HostSpecies:Chicken

SpeciesReactivity:Human,Mouse,Rat

Applications:ICC,IHC,WB

ProteinName/Synonyms:2",3"-cyclic-nucleotide3"-phosphodiesterase,CNP,CNPase,EC3.1.4.37

GeneID:CNP

AntibodyRegistryID(RRID):AB_2313538

PhysicalState:Liquid

ValidationandApplicationNotes

MolecularWeight:47kDa

WesternBlotDilutionRange:1:500-1:1000

IHCDilutionRange:1:500-1:1000

QualityControl

Qualityassuranceanalysiswasperformedusingimmunohistochemistry(atadilutionof1:2000)usingfluorescein-labeledgoatanti-chickenIgY(1:500dilution,AvesLabsCat.#F-1005)asthesecondaryreagent.

Storage

Storeat4°Cinthedark.Undertheseconditions,theantibodiesshouldhaveashelflifeofatleast12months(providedtheyremainsterile).Donotfreezetheseantibodiesunlessyouwanttostorethemforlongerperiodsoftime.Note,however,thateachtimeanantibodypreparationisfrozen,abouthalfofitsbindingactivityislost.

NOTE

AvesLabsproductsareintendedforuseasresearchlaboratoryreagents.Theyarenotintendedforuseasdiagnosticortherapeuticreagentsinhumans.

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