Details
GoatswereimmunizedwithpurifiedchickenIgY(wholeprotein)emulsifiedinFreund’sadjuvant.Aftermultipleinjections,goatswerebledandserumcollected.Antibodieswereisolatedusingaffinitychromatographyandconjugatedtoalkalinephosphatase.
Volume:1mL
Concentration:0.1mg/mL
Clonality:Polyclonal
Form:AffinityPurified
HostSpecies:Goat
SpeciesReactivity:Chicken
Immunogen:IgY
PhysicalState:Liquid
Conjugate:AP
Buffer:HEPES(50mM,pH7.0),NaCl(0.1M),bovineserumalbumin(0.2%,w/v),MgCl2(1.0mM),ZnCl2(0.1mM),sodiumazide(0.02%,w/v)(asanantimicrobialagent).
Specificity:Doesnotcross-reactwithmammalianIgGs
QualityControl
Antibodieswereanalyzedusingimmunoelectrophoresis(seebelow)andasandwichELISA.Inthislattertest,a1:800dilutionofGGHL-30PH-2wasfoundtogenerateanO.Dof1.0ina20minutereactionwithp-nitrophenylphosphateasthesubstrate.
Storage
Storeat4°Cinthedark.Undertheseconditions,theantibodiesshouldhaveashelflifeofatleast12months(providedtheyremainsterile).Forlongerstorageperiods,dilutetheantibodieswithsterileglycerolandstoreat-20°C.Donotfreezetheseantibodiesunlessyouwanttostorethemforlongerperiodsoftime.
NOTE
AvesLabsproductsareintendedforuseasresearchlaboratoryreagents.Theyarenotintendedforuseasdiagnosticortherapeuticreagentsinhumans.
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